Journal: Frontiers in Microbiology

Article Title: Potential Survival and Pathogenesis of a Novel Strain, Vibrio parahaemolyticus FORC_022, Isolated From a Soy Sauce Marinated Crab by Genome and Transcriptome Analyses

doi: 10.3389/fmicb.2018.01504

Figure Lengend Snippet: Cytotoxicity analyses of the FORC_022 strain were compared with the KCTC 2472 strain by measuring the activity of cytoplasmic lactate dehydrogenase (LDH). INT-407 cells were infected with FORC_022 or KCTC 2471 at various multiplicities of infection (MOIs) for (A) 2 h and (B) 3 h. Cytotoxicity was determined as the percentage of LDH leakage using the amount of LDH from the cells that were completely lysed by 2% Triton X-100. Error bars represent the standard errors of the means.

Article Snippet: For example, V. parahaemolyticus KCTC 2471 (=ATCC 33844 = CDC strain KC 824), which harbored tdh gene as major virulence factor, caused food poisoning in Japan ( ; ).

Techniques: Activity Assay, Infection

Journal: Annals of Microbiology

Article Title: Rapid identification and detection of Vibrio parahaemolyticus via different types of modus operandi with LAMP method in vivo

doi: 10.1186/s13213-020-01585-6

Figure Lengend Snippet: Fig. 1 V. parahaemolyticus detection in the localized infection mouse models. a The representative of V. parahaemolyticus proliferation identified on the specific TCBS plates from skin and MPB of localized infected mice. b The representative of V. parahaemolyticus detection using rapid LAMP assay from skin and MPB of localized infected mice. White arrowhead, representative sparse colonies of V. parahaemolyticus; P.C., positive control using plasmid containing target gene of V. parahaemolyticus as the template of LAMP reaction; N.C., negative control using ddH2O as the template of LAMP reaction; N.C. group, mice injected with PBS as the negative control of localized infection mouse models

Article Snippet: White arrowhead, representative sparse colonies of V. parahaemolyticus; P.C., positive control using plasmid containing target gene of V. parahaemolyticus as the template of LAMP reaction; N.C., negative control using ddH2O as the template of LAMP reaction; N.C. group, mice injected with PBS as the negative control of localized infection mouse models The LAMP assay indicated that the major organs from mice infected with all experimental foodborne strains exhibited “S”-shaped curves when mice were infected with V. parahaemolyticus through general infection (Fig. 4b, Table 5), while the small intestine and spleen were the most sensitive target organs in mice infected with the marine strain Vp 1A10122 or type strain Vp ATCC 17802.

Techniques: Infection, Lamp Assay, Positive Control, Plasmid Preparation, Negative Control, Injection

Journal: Annals of Microbiology

Article Title: Rapid identification and detection of Vibrio parahaemolyticus via different types of modus operandi with LAMP method in vivo

doi: 10.1186/s13213-020-01585-6

Figure Lengend Snippet: Fig. 2 V. parahaemolyticus detection in the foodborne infection mouse models. a The representative of V. parahaemolyticus proliferation identified on the specific TCBS plates from the stomach, small intestine, liver, kidney, spleen, and MPB of foodborne infected mice. b The representative of V. parahaemolyticus detection using rapid LAMP assay from the stomach, small intestine, liver, kidney, spleen, and MPB of foodborne-infected mice. White arrowhead, representative sparse colonies of V. parahaemolyticus; P.C., positive control using plasmid-containing target gene of V. parahaemolyticus as the template of LAMP reaction; N.C., negative control using ddH2O as the template of LAMP reaction; N.C. group, mice injected with PBS as the negative control of foodborne infection mouse models

Article Snippet: White arrowhead, representative sparse colonies of V. parahaemolyticus; P.C., positive control using plasmid containing target gene of V. parahaemolyticus as the template of LAMP reaction; N.C., negative control using ddH2O as the template of LAMP reaction; N.C. group, mice injected with PBS as the negative control of localized infection mouse models The LAMP assay indicated that the major organs from mice infected with all experimental foodborne strains exhibited “S”-shaped curves when mice were infected with V. parahaemolyticus through general infection (Fig. 4b, Table 5), while the small intestine and spleen were the most sensitive target organs in mice infected with the marine strain Vp 1A10122 or type strain Vp ATCC 17802.

Techniques: Infection, Lamp Assay, Positive Control, Plasmid Preparation, Negative Control, Injection

Journal: Annals of Microbiology

Article Title: Rapid identification and detection of Vibrio parahaemolyticus via different types of modus operandi with LAMP method in vivo

doi: 10.1186/s13213-020-01585-6

Figure Lengend Snippet: Fig. 3 V. parahaemolyticus detection in the wound infection mouse models. a The representative of V. parahaemolyticus proliferation identified on the specific TCBS plates from the stomach, small intestine, liver, kidney, spleen, and MPB of wound-infected mice; b The representative of V. parahaemolyticus detection using rapid LAMP assay from the stomach, small intestine, liver, kidney, spleen, and MPB of wound-infected mice. P.C., positive control using plasmid-containing target gene of V. parahaemolyticus as the template of LAMP reaction; N.C., negative control using ddH2O as the template of LAMP reaction; N.C. group, mice injected with PBS as the negative control of wound infection mouse models

Article Snippet: White arrowhead, representative sparse colonies of V. parahaemolyticus; P.C., positive control using plasmid containing target gene of V. parahaemolyticus as the template of LAMP reaction; N.C., negative control using ddH2O as the template of LAMP reaction; N.C. group, mice injected with PBS as the negative control of localized infection mouse models The LAMP assay indicated that the major organs from mice infected with all experimental foodborne strains exhibited “S”-shaped curves when mice were infected with V. parahaemolyticus through general infection (Fig. 4b, Table 5), while the small intestine and spleen were the most sensitive target organs in mice infected with the marine strain Vp 1A10122 or type strain Vp ATCC 17802.

Techniques: Infection, Lamp Assay, Positive Control, Plasmid Preparation, Negative Control, Injection

Journal: Annals of Microbiology

Article Title: Rapid identification and detection of Vibrio parahaemolyticus via different types of modus operandi with LAMP method in vivo

doi: 10.1186/s13213-020-01585-6

Figure Lengend Snippet: Fig. 4 V. parahaemolyticus detection in the general infection mouse models. a The representative of V. parahaemolyticus proliferation identified on the specific TCBS plates from the stomach, small intestine, liver, kidney, and spleen of general infected mice; b The representative of V. parahaemolyticus detection using rapid LAMP assay from the stomach, small intestine, liver, kidney, and spleen of general infected mice. White arrowhead, representative sparse colonies of V. parahaemolyticus; P.C., positive control using plasmid-containing target gene of V. parahaemolyticus as the template of LAMP reaction; N.C., negative control using ddH2O as the template of LAMP reaction; N.C. group, mice injected with PBS as the negative control of general infection mouse models

Article Snippet: White arrowhead, representative sparse colonies of V. parahaemolyticus; P.C., positive control using plasmid containing target gene of V. parahaemolyticus as the template of LAMP reaction; N.C., negative control using ddH2O as the template of LAMP reaction; N.C. group, mice injected with PBS as the negative control of localized infection mouse models The LAMP assay indicated that the major organs from mice infected with all experimental foodborne strains exhibited “S”-shaped curves when mice were infected with V. parahaemolyticus through general infection (Fig. 4b, Table 5), while the small intestine and spleen were the most sensitive target organs in mice infected with the marine strain Vp 1A10122 or type strain Vp ATCC 17802.

Techniques: Infection, Lamp Assay, Positive Control, Plasmid Preparation, Negative Control, Injection

Journal: Annals of Microbiology

Article Title: Rapid identification and detection of Vibrio parahaemolyticus via different types of modus operandi with LAMP method in vivo

doi: 10.1186/s13213-020-01585-6

Figure Lengend Snippet: Fig. 5 V. parahaemolyticus direct detection from fresh MPB of infected mice via different infection routes. a The representative of V. parahaemolyticus proliferation identified on the specific TCBS plates from fresh MPB of wound-infected mice; b The representative of V. parahaemolyticus detection using rapid LAMP assay from fresh MPB of localized, foodborne-derived, and wound infection mice. P.C., positive control using plasmid containing target gene of V. parahaemolyticus as the template of LAMP reaction; N.C., negative control using ddH2O as the template of LAMP reaction; N.C. group, mice injected with PBS as the negative control of general infection mouse models

Article Snippet: White arrowhead, representative sparse colonies of V. parahaemolyticus; P.C., positive control using plasmid containing target gene of V. parahaemolyticus as the template of LAMP reaction; N.C., negative control using ddH2O as the template of LAMP reaction; N.C. group, mice injected with PBS as the negative control of localized infection mouse models The LAMP assay indicated that the major organs from mice infected with all experimental foodborne strains exhibited “S”-shaped curves when mice were infected with V. parahaemolyticus through general infection (Fig. 4b, Table 5), while the small intestine and spleen were the most sensitive target organs in mice infected with the marine strain Vp 1A10122 or type strain Vp ATCC 17802.

Techniques: Infection, Lamp Assay, Derivative Assay, Positive Control, Plasmid Preparation, Negative Control, Injection